Biotechnology procedures and experiments hand book / (Record no. 8914)
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| 000 -LEADER | |
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| fixed length control field | 12216nam a22001577a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20210423132916.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210423b ||||| |||| 00| 0 eng d |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | e-book (FST) |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Harisha, S. |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Subordinate unit | |
| 245 ## - TITLE STATEMENT | |
| Title | Biotechnology procedures and experiments hand book / |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | New Delhi, India : |
| Name of publisher, distributor, etc | Infinity science press, |
| Date of publication, distribution, etc | 2007. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc | CONTENTS<br/>Chapter 1. General Instruction and Laboratory Methods 1<br/>General Instruction 1<br/>General Laboratory Methods 2<br/>Chapter 2. Tools and Techniques in Biological Studies 9<br/>Spectrophotometry 9<br/>Electrophoresis 13<br/>Column Chromatography 18<br/>pH Meter 22<br/>Centrifugation 24<br/>Ultrasonic Cell Disruption 29<br/>Conductivity Meter 32<br/>Radioactive Tracers 32<br/>Autoradiography 37<br/>Photography 39<br/>Statistics 41<br/>Graphs 44<br/>Computers 47<br/>Chapter 3. Biochemistry 51<br/>Carbohydrates 51<br/>Exercise 1. Qualitative Tests 52<br/>Exercise 2. Qualitative Analysis 54<br/>Exercise 3. Estimate the Amount of Reducing Sugars 56<br/>Exercise 4. Estimation of Reducing Sugar by Somogyi’s Method 59<br/>Exercise 5. Estimation of Sugar by Folin-Wu Method 60<br/>vii<br/>viii CONTENTS<br/>Exercise 6. Estimation of Sugar by Hagedorn-Jenson Method 61<br/>Exercise 7. Estimation of Reducing Sugars by the Dinitro<br/>Salicylic Acid (DNS) Method 62<br/>Exercise 8. Determination of Blood Glucose by Hagedorn-Jenson<br/>Method 63<br/>Exercise 9. Determining Blood Sugar by Nelson and<br/>Somogyi’s Method 65<br/>Exercise 10. Determination of Blood Glucose by the O-Toluidine<br/>Method 66<br/>Exercise 11. Estimation of Protein by the Biuret Method 67<br/>Exercise 12. Estimation of Protein by the FC-Method 68<br/>Exercise 13. Protein Assay by Bradford Method 69<br/>Exercise 14. Estimation of Protein by the Lowry Protein Assay 70<br/>Exercise 15. Biuret Protein Assay 71<br/>Exercise 16. Estimation of DNA by the Diphenylamine Method 72<br/>Exercise 17. Estimation of RNA by the Orcinol Method 73<br/>Chapter 4. Enzymology 75<br/>Enzymes 75<br/>Exercise 1. Demonstrating the Presence of Catalase in Pig’s Liver 83<br/>Exercise 2. Determining the Optimum pH for Trypsin 84<br/>Exercise 3. Extraction of Tyrosinase 85<br/>Exercise 4. Preparation of Standard Curve 87<br/>Exercise 5. Enzyme Concentration 89<br/>Exercise 6. Effects of pH 90<br/>Exercise 7. Effects of Temperature 91<br/>Exercise 8. Computer Simulation of Enzyme Activity 92<br/>Exercise 9. Kinetic Analysis 93<br/>Exercise 10. Determination of Km and Vmax 94<br/>Exercise 11. Addition of Enzyme Inhibitors 95<br/>Exercise 12. Protein Concentration/Enzyme Activity 97<br/>Exercise 13. Studying the Action and Activity of Amylase<br/>on Starch Digestion 98<br/>Exercise 14. Determination of the Effect of pH on the<br/>Activity of Human Salivary a-Amylase 99<br/>CONTENTS ix<br/>Exercise 15. Determining the Effect of Temperature on the<br/>Activity of Human Salivary a-Amylase 101<br/>Exercise 16. Construction of the Maltose Calibration Curve 102<br/>Chapter 5. Electrophoresis 103<br/>Electrophoresis 103<br/>Exercise 1. Preparation of SDS-Polyacrylamide Gels 108<br/>Exercise 2. Separation of Protein Standards: SDS-PAGE 110<br/>Exercise 3. Coomassie Blue Staining of Protein Gels 111<br/>Exercise 4. Silver Staining of Gels 112<br/>Exercise 5. Documentation 114<br/>Exercise 6. Western Blots 114<br/>Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis<br/>(SDS-PAGE) 117<br/>Preparation of Polyacrylamide Gels 119<br/>Agarose Gel Electrophoresis 121<br/>Agarose Gel Electrophoresis 123<br/>Chapter 6. Microbiology 125<br/>Introduction 125<br/>The Microscopy 127<br/>Exercise 1. The Bright Field Microscope 134<br/>Exercise 2. Introduction to the Microscope and Comparison<br/>of Sizes and Shapes of Microorganisms 137<br/>Exercise 3. Cell Size Measurements: Ocular and<br/>Stage Micrometers 146<br/>Exercise 4. Measuring Depth 147<br/>Exercise 5. Measuring Area 148<br/>Exercise 6. Cell Count by Hemocytometer or Measuring Volume 149<br/>Exercise 7. Measurement of Cell Organelles 152<br/>Exercise 8. Use of Darkfield Illumination 152<br/>Exercise 9. The Phase Contrast Microscope 153<br/>Exercise 10. The Inverted Phase Microscope 155<br/>Aseptic Technique and Transfer of Microorganisms 155<br/>Control of Microorganisms by using Physical Agents 163<br/>x CONTENTS<br/>Control of Microorganisms by using Disinfectants and<br/>Antiseptics 172<br/>Control of Microorganisms by using Antimicrobial Chemotherapy 180<br/>Isolation of Pure Cultures from a Mixed Population 191<br/>Bacterial Staining 201<br/>Direct Stain and Indirect Stain 206<br/>Gram Stain and Capsule Stain 211<br/>Endospore Staining and Bacterial Motility 215<br/>Enumeration of Microorganisms 221<br/>Biochemical Test for Identification of Bacteria 233<br/>Triple Sugar Iron Test 241<br/>Starch Hydrolysis Test (II Method) 242<br/>Gelatin Hydrolysis Test 244<br/>Catalase Test 245<br/>Oxidase Test 247<br/>IMVIC Test 249<br/>Extraction of Bacterial DNA 257<br/>Medically Significant Gram–Positive Cocci (GPC) 259<br/>Protozoans, Fungi, and Animal Parasites 263<br/>The Fungi, Part 1–The Yeasts 264<br/>Performance Objectives 267<br/>The Fungi, Part 2—The Molds 269<br/>Viruses: The Bacteriophages 274<br/>Serology, Part 1–Direct Serologic Testing 279<br/>Serology, Part 2–Indirect Serologic Testing 289<br/>Chapter 7. Cell Biology and Genetics 299<br/>Cell Cycles 299<br/>Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain 306<br/>Meiosis in Grasshopper Testis (Poecilocerus Pictus) 309<br/>Mitosis in Onion Root Tip (Allium Cepa) 311<br/>Differential Staining of Blood 313<br/>Buccal Epithelial Smear and Barr Body 315<br/>Vital Staining of DNA and RNA in Paramecium 316<br/>CONTENTS xi<br/>Induction of Polyploidy 317<br/>Mounting of Genitalia in Drosophila Melanogaster 318<br/>Mounting of Genitalia in the Silk Moth Bombyx Mori 319<br/>Mounting of the Sex Comb in Drosophila Melanogaster 320<br/>Mounting of the Mouth Parts of the Mosquito 321<br/>Normal Human Karyotyping 322<br/>Karyotyping 322<br/>Black and White Film Development and Printing for Karyotype<br/>Analysis 324<br/>Study of Drumsticks in the Neutrophils of Females 327<br/>Study of the Malaria Parasite 328<br/>Vital Staining of DNA and RNA in Paramecium 330<br/>Sex-Linked Inheritance in Drosophila Melanogaster 331<br/>Preparation of Somatic Chromosomes from Rat Bone Marrow 333<br/>Chromosomal Aberrations 334<br/>Study of Phenocopy 336<br/>Study of Mendelian Traits 337<br/>Estimation of Number of Erythrocytes [RBC] in Human Blood 338<br/>Estimation of Number of Leucocytes (WBC) in Human Blood 340<br/>Culturing Techniques and Handling of Flies 342<br/>Life Cycle of the Mosquito (Culex Pipiens) 343<br/>Life Cycle of the Silkworm (Bombyx Mori) 344<br/>Vital Staining of Earthworm Ovary 346<br/>Culturing and Observation of Paramecium 347<br/>Culturing and Staining of E.coli (Gram’s Staining) 348<br/>Breeding Experiments in Drosophila Melanogaster 349<br/>Preparation of Salivary Gland Chromosomes 354<br/>Observation of Mutants in Drosophila Melanogaster 355<br/>ABO Blood Grouping and Rh Factor in Humans 356<br/>Determination of Blood Group and Rh Factor 357<br/>Demonstration of the Law of Independent Assortment 358<br/>Demonstration of Law of Segregation 360<br/>xii CONTENTS<br/>Chapter 8. Molecular Biology 363<br/>The Central Dogma 363<br/>Exercise 1. Protein Synthesis in Cell Free Systems 364<br/>Chromosomes 366<br/>Exercise 2. Polytene Chromosomes of Dipterans 367<br/>Exercise 3. Salivary Gland Preparation (Squash Technique) 368<br/>Exercise 4. Extraction of Chromatin 369<br/>Exercise 5. Chromatin Electrophoresis 371<br/>Exercise 6. Extraction and Electrophoresis of Histones 372<br/>Exercise 7. Karyotype Analysis 375<br/>Exercise 8. In Situ Hybridization 378<br/>Exercise 9. Culturing Peripheral Blood Lymphocytes 380<br/>Exercise 10. Microslide Preparation of Metaphases for<br/>In-Situ Hybridization 382<br/>Exercise 11. Staining Chromosomes (G-Banding) 384<br/>Nucleic Acids 386<br/>Exercise 12. Extraction of DNA from Bovine Spleen 387<br/>Exercise 13. Purification of DNA 388<br/>Exercise 14. Characterization of DNA 389<br/>Exercise 15. DNA-Dische Diphenylamine Determination 390<br/>Exercise 16. Melting Point Determination 391<br/>Exercise 17. CsCl-Density Separation of DNA 393<br/>Exercise 18. Phenol Extraction of rRNA (Rat liver) 394<br/>Exercise 19. Spectrophotometric Analysis of rRNA 395<br/>Exercise 20. Determination of Amount of RNA by the<br/>Orcinol Method 396<br/>Exercise 21. Sucrose Density Fractionation 397<br/>Exercise 22. Nucleotide Composition of RNA 398<br/>Isolation of Genomic DNA—DNA Extraction Procedure 400<br/>Exercise 23. Isolation of Genomic DNA from Bacterial Cells 401<br/>Exercise 24. Preparation of Genomic DNA from Bacteria 404<br/>Exercise 25. Extraction of Genomic DNA from Plant Source 405<br/>Exercise 26. Extraction of DNA from Goat Liver 407<br/>Exercise 27. Isolation of Cotton Genomic DNA from Leaf Tissue 408<br/>CONTENTS xiii<br/>Exercise 28. Arabidopsis Thaliana DNA Isolation 411<br/>Exercise 29. Plant DNA Extraction 412<br/>Exercise 30. Phenol/Chloroform Extraction of DNA 414<br/>Exercise 31. Ethanol Precipitation of DNA 414<br/>Exercise 32. Isolation of Mitochondrial DNA 415<br/>Exercise 33. Isolation of Chloroplast DNA 417<br/>Exercise 34. DNA Extraction of Rhizobium (CsCl Method) 417<br/>Exercise 35. Isolation of Plasmids 418<br/>Exercise 36. RNA Isolation 422<br/>Preparation of Vanadyl-Ribonucleoside Complexes<br/>that Inhibit Ribonuclease Activity 422<br/>Exercise 37. RNA Extraction Method for Cotton 423<br/>Exercise 38. Isolation of RNA from Bacteroids 424<br/>Exercise 39. Isolation of RNA from Free-Living Rhizobia 425<br/>Estimation of DNA purity and Quantification 426<br/>Exercise 40. Fungal DNA Isolation 427<br/>Exercise 41. Methylene Blue DNA Staining 429<br/>Exercise 42. Transformation 431<br/>Blotting Techniques—Southern, Northern, Western Blotting 433<br/>Preparing the Probe 440<br/>Exercise 43. Southern Blotting (First Method) 444<br/>Exercise 44. Southern Blotting (Second Method) 446<br/>Exercise 45. Western Blotting 447<br/>Exercise 46. Western Blot Analysis of Epitoped-tagged Proteins<br/>using the Chemifluorescent Detection Method for<br/>Alkaline Phosphatase-conjugated Antibodies 450<br/>Southern Blot 452<br/>Exercise 47. Southern Analysis of Mouse Toe/Tail DNA 453<br/>Exercise 48. Northern Blotting 454<br/>Restriction Digestion Methods—Restriction Enzyme Digests 457<br/>Exercise 49. Restriction Digestion of Plasmid, Cosmid, and<br/>Phage DNAs 460<br/>xiv CONTENTS<br/>Exercise 50. Manual Method of Restriction Digestion of<br/>Human DNA 461<br/>Exercise 51. Preparation of High-Molecular-Weight Human<br/>DNA Restriction Fragments in Agarose Plugs 463<br/>Exercise 52. Restriction Enzyme Digestion of DNA 465<br/>Exercise 53. Electroelution of DNA Fragments from Agarose<br/>into Dialysis Tubing 466<br/>Exercise 54. Isolation of Restriction Fragments from Agarose<br/>Gels by Collection onto DEAE Cellulose 469<br/>Exercise 55. Ligation of Insert DNA to Vector DNA 471<br/>PCR Methods (Polymerase Chain Reaction) 472<br/>Exercise 56. Polymerase Chain Reaction 475<br/>Exercise 57. DNA Amplification by the PCR Method 476<br/>Chapter 9. Tissue Culture Techniques 477<br/>Tissue Culture Methods 477<br/>Plant Tissue Culture 487<br/>Plant Tissue Culture 491<br/>Many Dimensions of Plant Tissue Culture Research 493<br/>What is Plant Tissue Culture? 496<br/>Uses of Plant Tissue Culture 497<br/>Plant Tissue Culture demonstration by Using<br/>Somaclonal Variation to Select for Disease Resistance 498<br/>Demonstration of Tissue Culture for Teaching 499<br/>Preparation of Plant Tissue Culture Media 502<br/>Plant Tissue Culture Media 505<br/>Preparation of Protoplasts 512<br/>Protoplast Isolation, Culture, and Fusion 514<br/>Agrobacterium Culture and Agrobacterium—<br/>Mediated transformation 518<br/>Isolation of Chloroplasts from Spinach Leaves 520<br/>Preparation of Plant DNA using CTAB 522<br/>Suspension Culture and Production of Secondary Metabolites 525<br/>Protocols for Plant Tissue Culture 529<br/>Sterile Methods in Plant Tissue Culture 535<br/>CONTENTS xv<br/>Media for Plant Tissue Culture 538<br/>Safety in Plant Tissue Culture 543<br/>Preparation of Media for Animal Cell Culture 544<br/>Aseptic Technique 546<br/>Culture and Maintenance of Cell Lines 550<br/>Trypsinizing and Subculturing Cells from a Monolayer 557<br/>Cellular Biology Techniques 558<br/>In Vitro Methods 574<br/>Human Cell Culture Methods 577<br/>Chapter 10. Tests 595<br/>Starch Hydrolysis Test 595<br/>Gelatin Hydrolysis Test 597<br/>Catalase Test 598<br/>Oxidase Test 599<br/>Appendix A (Units and Measures) 603<br/>Appendix B (Chemical Preparations) 617<br/>Appendix C (Reagents Required for Tissue<br/>Culture Experiments) 645<br/>Appendix D (Chemicals Required for Microbiology<br/>Experiments) 671<br/>Appendix E (About the CD-ROM) 689<br/>Index 691 |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bamforth, Charles W |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | E-Book |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total Checkouts | Full call number | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | College of Natural Resources | College of Natural Resources | 23/04/2021 | e-book (FST) | 23/04/2021 | 23/04/2021 | E-Book |